Effects of in vivo exposures to nanoparticles (Al<sub>2</sub>O<sub>3</sub>, CuO, TiO<sub>2</sub>) on the activities of ATPases in the gill and muscle of freshwater mussel (Unio tigridis)

Mussels are effective bioindicator organisms for aquatic environments. Therefore, they were often used to determine the effects of various xenobiotics in systems. There is no study our knowledge on vivo nanoparticles (NPs) activities ATPases freshwater mussels (Unio tigridis). This work demonstrates Al$_{2}$O$_{3}$, CuO, and TiO$_{2}$ NPs Na- ATPase, Ca-ATPase, Mg-ATPase gill muscle following 14-day exposures different concentrations (0, 1, 3, 9 mg/L). fed with laboratory cultured algae (Chlorella vulgaris) during exposures. was ouabainsensitive ATPase activity tissues U. tigridis. The Na-ATPase Ca-ATPase decreased significantly (p < 0.05), while increased. However, all after NP muscle. more significant alterations compared order determined as > CuO Al$_{2}$O$_{3}$. alteration consumption between control NP-exposed mussels. present reporting first data tigridis demonstrated sensitivity mussel physiology towards exposures, suggesting further studies understand better physiological response